Peptide synthesis catalyzed by immobilized trypsin in organic solvents

YEŞİLOĞLU Y.

Journal of the Chemical Society of Pakistan, cilt.26, sa.3, ss.244-247, 2004 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 26 Sayı: 3
  • Basım Tarihi: 2004
  • Dergi Adı: Journal of the Chemical Society of Pakistan
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.244-247
  • Trakya Üniversitesi Adresli: Evet

Özet

Trypsin was immobilized to poly vinyl alcohol (PVA) by adsorption from its aqueous solutions. The catalytic activity of trypsin increased markedly by immobilization for peptide synthesis from N-α-benzoyl L-arginine in hydrophilic organic solvents such as tetrahydrofuran or ethanol. The yield of Bz-Arg-Leu-NH2 is strongly dependent on the PVA/trypsin ratio and water content in the reaction medium. The optimum pH and temperature were determined for immobilized trypsin as 7.0 and 40°C, respectively.