The effect of platelet-rich plasma in inactive form on the burn zone of stasis in rats
Journal of Plastic Surgery and Hand Surgery, cilt.53, sa.5, ss.301-308, 2019 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 53 Sayı: 5
- Basım Tarihi: 2019
- Doi Numarası: 10.1080/2000656x.2019.1614454
- Dergi Adı: Journal of Plastic Surgery and Hand Surgery
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.301-308
- Anahtar Kelimeler: Burn, platelet rich plasma, zone of stasis
- Trakya Üniversitesi Adresli: Evet
Özet
The protection of the burn stasis zone tissues (BSZT) reduces the width and depth of the burn injury. In this study, it is aimed to show the effect of platelet-rich plasma (PRP) on the burn zone of stasis. Seventy-two Wistar rats were used in the study. PRP was obtained from the blood taken from eight rats. The remaining 64 rats were divided into four groups. In Group 1, only the burn procedure was performed. In Group 2, 0.3 cc of physiological saline solution, in Group 3, 0.3 cc of platelet-poor plasma and in Group 4, 0.3 cc of PRP were intradermally injected into BSZT after burn procedure. 21.5% of the tissues in Group 1, 20.8% in Group 2, 27.0% in Group 3, and 69.6% in Group 4 were found to be alive. The autophagic cell number average was calculated as 340 in Group 1, 340 in Group 2, 335 in Group 3 and 450 in Group 4, while the average number of cells stained with Nrf2 was calculated as 225 in Group 1, 245 in Group 2, 250 in Group 3 and 370 in Group 4. When the groups were compared in terms of the living tissue ratio, autophagy and number of cells stained with Nrf2, the values in Group 4 were found to be statistically significantly higher compared to Group 1, Group 2 and Group 3, while there was no difference between Groups 1, 2 and 3. This study has shown that PRP has a protective effect on BSZT.