Akademik Veri Yönetim Sistemi
CURRENT ISSUES IN MOLECULAR BIOLOGY, cilt.48, sa.1, ss.48-53, 2025 (SCI-Expanded, Scopus)
In our study, we aimed to investigate the relationship between miRNA-21, miRNA-155,
miRNA-34a, IL-6, TGF-β, and TNF-α expression levels and serum trace element levels
in the development and progression of bladder cancer. RT-PCR was used to establish
miRNA-21, miRNA-155, and miRNA-34a expression levels while serum IL-6, TGF-β, and
TNF-α levels were determined using the ELISA and measured with an atomic absorption
spectrophotometer. In the patient group, miRNA-21 and miRNA-155 expression levels
were significantly higher compared with the healthy control group (p < 0.001). Furthermore,
in the patient group, miRNA-34a expression was significantly lower compared with the
control group (p < 0.001). IL-6, TNF-α, TGF-β, copper levels, and the copper to zinc ratio
were significantly higher in the patient group (p < 0.001). Serum iron and zinc levels in the
patient group were significantly lower compared with the control group (p < 0.001). There
was a significant positive correlation between miRNA-155 and IL-6 and TNF-α (r = 0.279,
p = 0.015*; r = 0.325**, p = 0.004). A significant positive correlation was detected between
miRNA-34a and IL-6 and TGF-β (r = 0.294*, p = 0.010; r = 0.447**, p < 0.001). By evaluating
these important biomarkers together, it might be possible to implement clinical applications
for bladder cancer treatment and develop individual therapeutic strategies.