Endocannabinoid and n-acylethanolamide levels in rat brain and spinal cord following systemic dipyrone and paracetamol administration

TOPUZ R. D., GÜNDÜZ Ö., KARADAĞ Ç. H., DÖKMECİ D., ULUGÖL A.

Canadian Journal of Physiology and Pharmacology, cilt.97, sa.11, ss.1035-1041, 2019 (SCI-Expanded, Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 97 Sayı: 11
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1139/cjpp-2019-0015
  • Dergi Adı: Canadian Journal of Physiology and Pharmacology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1035-1041
  • Anahtar Kelimeler: Dipyrone, Endocannabinoid, N-acylethanolamide, Paracetamol
  • Trakya Üniversitesi Adresli: Evet

Özet

The cannabinoid system has been suspected to play a role in the mechanisms of action of dipyrone and paracetamol. Our purpose was to measure the local endocannabinoid and N-acylethanolamide levels in the brain and spinal cord of rats following dipyrone and paracetamol administration. Nociception was assessed 1, 5, and 12 h following drug injections in Wistar rats, using tail-flick and hot-plate tests. The antinociceptive effects of dipyrone (150, 300, and 600 mg/kg, i.p.) and paracetamol (30, 100, and 300 mg/kg, i.p.) were observed. After administration of the highest doses of dipyrone and paracetamol, endocannabinoid (N-arachidonoylethanolamide (AEA), 2-arachidonoylglycerol (2-AG)) and N-acylethanolamide (palmitoylethanolamide (PEA), oleoylethanolamide (OEA)) levels were measured in the periaqueductal gray (PAG), rostral ventromedial medulla (RVM), and spinal cords of rats using tandem mass spectrometry with liquid chromatography. Increased 2-AG levels were observed in the PAG and the RVM 12 h after paracetamol injection; dipyrone exerted no action on 2-AG levels. Analgesic administrations led to a reduction in AEA levels in the RVM and spinal cord; similar decreases in PEA and OEA levels were observed in the RVM and the spinal cord. Dipyrone and paracetamol administrations appear to exert complicated effects on endocannabinoid and N-acylethanolamide levels in rats.