Exogenous l-arginine and HDL can alter LDL and ox-LDL-mediated platelet activation: Using platelet P-selectin receptor numbers
Clinical and Applied Thrombosis/Hemostasis, cilt.17, sa.6, 2011 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 17 Sayı: 6
- Basım Tarihi: 2011
- Doi Numarası: 10.1177/1076029610385223
- Dergi Adı: Clinical and Applied Thrombosis/Hemostasis
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Anahtar Kelimeler: high density lipoprotein, l-arginine, low-density lipoprotein, nitric oxide, oxidized low-density lipoprotein, P-selectin, platelet
- Trakya Üniversitesi Adresli: Evet
Özet
The aim of this study is to investigate the effects of exogenous l-arginine and HDL on LDL and oxidized LDL (ox-LDL)-mediated platelet activation. Adenosine diphosphate (ADP)-activated platelets have been incubated with lipoproteins with or without l-arginine. P-selectin receptor numbers per platelet have been measured by flow cytometry. After incubation with only l-arginine (without lipoproteins), platelet nitric oxide (NO) levels and P-selectin receptor numbers significantly increased compared to the controls (P <.05). After incubation with LDL or ox-LDL, receptor numbers of P-selectin significantly increased (P <.001). However, P-selectin receptor numbers in platelets treated with l-arginine + LDL or l-arginine + ox-LDL decreased compared to the levels in platelets treated with only LDL or ox-LDL (P <.01, P <.001, respectively). Addition of HDL to l-arginine + ox-LDL caused significant reduction in P-selectin receptor numbers as in the control values (P <.001).We have concluded that l-arginine causes enhanced platelet NO levels and blocks the effects of LDL or ox-LDL on platelet P-selectin receptor numbers and HDL also strengthens this effect of l-arginine. © SAGE Publications 2011.