Akademik Veri Yönetim Sistemi
Journal of Food Composition and Analysis, cilt.134, 2024 (SCI-Expanded, Scopus)
In this study, a laccase-based amperometric biosensor was fabricated for the quantitative analysis of total phenolic content in Hardaliye. The biosensor was constructed using a carbon paste electrode with simple modifications. The laccase obtained from Trametes versicolor was immobilized into a carbon paste electrode modified with a gelatin matrix. The immobilization parameters for biosensor fabrication, as well as its working conditions were optimized with gallic acid as the phenolic standard. Additionally, molecular docking simulation was used to examine the interaction between Trametes versicolor laccase and gallic acid. The biosensor's analytical parameters were determined under optimal conditions including linearity, repeatability, stability and selectivity. The linear range of gallic acid was calculated as 0.5–100 μM, with the detection limit of 0.1323 ± 0.1 μM. With good selectivity and stability, the biosensor successfully determined the total phenolic contents in Hardaliye, and red-wine samples. Comparison studies showed that the biosensor results agreed with those obtained from the Liquid Chromatography-Tandem Mass Spectrometry method. This work indicates that the proposed biosensor is a promising analytic tool for total phenolic analyses of beverages.