Characterization of calpastatin gene in purebred and crossbred turkish grey steppe cattle Safkan ve melez Türk boz irk sıǧırlarda Kalpastatin geninin Karakterizasyonu
Kafkas Universitesi Veteriner Fakultesi Dergisi, cilt.19, sa.2, ss.203-206, 2013 (SCI-Expanded, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 19 Sayı: 2
- Basım Tarihi: 2013
- Doi Numarası: 10.9775/kvfd.2012.7470
- Dergi Adı: Kafkas Universitesi Veteriner Fakultesi Dergisi
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.203-206
- Anahtar Kelimeler: Calpastatin (CAST), Genetic marker, Grey steppe cattle, PCR-RFLP, Polymorphism
- Trakya Üniversitesi Adresli: Evet
Özet
Calpastatin (CAST) is a specific inhibitor of calcium-dependent neutral protease μ-calpain found in mammalian tissues. The genetic variants in the bovine CAST gene were analysed by using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The animal material of the study consisted of 132 bulls and heifers in Turkish Grey steppe cattle and Turkish Grey steppe x Brown Swiss crossbreds. C and G alleles are characterized by CAST/RsaI polymorphism were detected in the sample of animals were studied. Allele frequency C was significantly frequent in the crossbred group compared to the pure Turkish Grey steppe animals (P<0.05). In the total samples of animals, the average allele frequency C was 56.1%. Genotypic frequencies were estimated as 0.257, 0.499 and 0.243 in the purebred Turkish Grey steppe, and 0.388, 0.470 and 0.142 in the Turkish Grey steppe x Brown Swiss crossbred population for genotypes CC, CG and GG, respectively. As a result, genotypic distributions were equilibrium in both pure and crossbred examples of Turkish Grey steppe cattle.