Enzymatic Synthesis of Prebiotic Carbohydrates from Lactose: Integration of Osmotic Membrane Distillation with Batch Reactor System

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Cinar K., GÜLEÇ H. A., ONSEKİZOĞLU BAĞCI P., BAĞCI U., Güneş G.

FOOD AND BIOPROCESS TECHNOLOGY, cilt.18, sa.1, ss.756-768, 2025 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 18 Sayı: 1
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1007/s11947-024-03479-w
  • Dergi Adı: FOOD AND BIOPROCESS TECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Compendex, INSPEC
  • Sayfa Sayıları: ss.756-768
  • Anahtar Kelimeler: Aspergillus oryzae, Galactooligosaccharide, Osmotic membrane distillation, Reactor design, Yield, β-galactosidase
  • Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
  • Trakya Üniversitesi Adresli: Evet

Özet

This study aimed to enhance galactooligosaccharide (GOS) yield by maintaining high lactose concentration levels using osmotic membrane distillation (OMD) throughout the synthesis. The main problem influencing GOS yield during transgalactosylation is the decrease of lactose concentration over extended reaction periods. By integrating OMD with a continuous stirred batch reactor, water was progressively removed from the reaction medium. Compared to a standard batch reactor, the OMD-integrated system not only increased GOS yield by a maximum of 20.1% but also reduced the time needed for equivalent lactose conversion about 15-90 min. This integration particularly boosted the formation of longer-chain GOS. A consistent GOS yield of 67% was achieved, with up to 28% lactose conversion, surpassing the non-integrated reactor's performance. The proposed reactor design shows promise for enhancing GOS production efficiency while concurrently concentrating the reaction medium in a single step.