The effects of onion (Allium cepa) extract on doxorubicin-induced apoptosis in aortic endothelial cells

Alpsoy S., Uygur R., Aktas C., Topcu B., KANTER M., Erboga M., ...More

Journal of Applied Toxicology, vol.33, no.5, pp.364-369, 2013 (SCI-Expanded, Scopus) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 33 Issue: 5
  • Publication Date: 2013
  • Doi Number: 10.1002/jat.1750
  • Journal Name: Journal of Applied Toxicology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.364-369
  • Keywords: Allium cepa, Antioxidants, Aortic endothelial cells, Doxorubicin, Rat, TUNEL
  • Trakya University Affiliated: Yes

Abstract

The aim of this study was to investigate the effects of onion (Allium cepa) extracts (ACE) on doxorubicin (DOX)-induced apoptosis in aortic endothelial cells. The rats in the ACE-pretreated group were given a daily dose of 1ml ACE for 14days. To induce aortic endothelial cell apoptosis, DOX (30mgkg-1 body weight) was injected intraperitoneally by a single dose and the rats were sacrificed after 48h. To date, no such studies have been performed on antiapoptotic potential of ACE on DOX-induced apoptosis in aortic endothelial cells. Our data indicate a significant reduction in the activity of in situ identification of apoptosis using terminal dUTP nick end-labeling in aortic endothelial cells of the DOX-treated group with ACE therapy. DOX-treated with ACE groups showed a significant decrease in malondialdehyde levels and increased levels of glutathione in comparison with the DOX-treated group. Data from our study show that prevention of endothelial cell apoptosis by ACE may contribute to the restoration of aortic endothelial dysfunction that is associated with DOX treatment. © 2011 John Wiley & Sons, Ltd.