Research Information System
Journal of International Dental and Medical Research, vol.9, no.3, pp.267-271, 2016 (Scopus)
The aim of the present study is to investigate mutations in tumor suppressor BRCA1 and BRCA2 genes and P53 gene polymorphism in high-voltage power station workers and to determine the distribution of this polymorphism among these workers. This study carried out on 35 high-voltage transmission line (HVTL) workers with a mean age of 36.6 ± 6.8 years. The data obtained from the exposure group were compared to the control group. Control group was created from volunteers who have same age range (35.4 ± 6.4 years) and socio-cultural level except occupational extremely low frequency magnetic field (ELFMF) exposure. DNAs were isolated from the blood samples collected from both groups and BRCA1 and BRCA2 gene mutations and p53 gene polymorphism were investigated. Isolated DNAs were used to amplify the mutation regions of exon 2 and exon 20 regions for BRCA1 and exon 2 for BRCA2 with polymerase chain reaction (PCR) and the single-strand conformational polymorphism (SSCP) method was used to detect the mutations. PCR and Restriction Fragment Length Polymorphism (RFLP) were used to determine P53 codon 72 Arg/Pro gene polymorphism. The exon 2 mutation of BRCA1 was detected in only 1 worker in the occupational exposure group. It should be noted that the worker was also cancer and receiving treatment. It remains unclear whether the mutation was cancer-related or a result of occupational exposure. However, the investigation on P53 gene polymorphism did not indicate any difference between the groups (p > 0.05). In conclusion, BRCA1 and BRCA2 mutations were not observed among the high-voltage transmission line workers except one in this study. However, P53 gene polymorphism also showed no difference between groups. Finally, further studies are required to clarify the underlying causes of the mutation detected in a worker in this study group.