FOOD TECHNOLOGY AND BIOTECHNOLOGY, cilt.45, sa.4, ss.439-442, 2007 (SCI-Expanded, Scopus)
Solid-state fermentation (SSF) was carried out using corncob leaf (CL), rye straw (RS), wheat straw (WS) and wheat bran (WB) as substrates for a-amylase production by a fungal culture of Penicillium chrysogenum. The effects of moisture level, particle size and inoculum concentration on enzyme synthesis from R chrysogenum were investigated. Optimal moisture levels of substrates were 75, 65, 65 and 55% for CL, WS, WB and RS substrates, respectively. Optimal particle size and inoculum concentration for the production of alpha-amylase were: >1 mm, 20%; >1 mm, 20%; 1 mm, 20% and >1 mm, 30% for CL, WS, WB and RS, respectively WB showed the highest enzyme production with 160 U/mL under optimum conditions. The other enzyme activities were 28, 49 and 45 U/mL using CL, RS and WS, respectively.