Investigation of the Relationship Between IL-18 (− 607 C/A), IL-18 (− 137 G/C), and MMP-2 (− 1306 C/T) Gene Variations and Serum Copper and Zinc Levels in Patients Diagnosed with Chronic Renal Failure

AY A., Alkanli N., ÜSTÜNDAĞ S.

Biological Trace Element Research, cilt.200, sa.5, ss.2040-2052, 2022 (SCI-Expanded, Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 200 Sayı: 5
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1007/s12011-021-02828-6
  • Dergi Adı: Biological Trace Element Research
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, Food Science & Technology Abstracts, MEDLINE, Pollution Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.2040-2052
  • Anahtar Kelimeler: Chronic renal failure, IL-18 (− 137 G/C) gene variation, IL-18 (− 607 C/A) gene variation, MMP-2 (− 1306 C/T) gene variation, PCR–RFLP, Trace elements
  • Trakya Üniversitesi Adresli: Evet

Özet

The aim of this study is to investigate the relationship between IL-18 (− 607 C/A), IL-18 (− 137 G/C), and MMP-2 (− 1306 C/T) gene variations and serum trace element levels in patients diagnosed with CRF. Genotype distributions of IL-18 (− 607 C/A, − 137 G/C) gene variations were determined by polymerase chain reaction (PCR) method. PCR-restriction fragment length polymorphism (RFLP) methods were used to determine the MMP-2 (− 1306 C/T) gene variation genotype distributions. Serum trace element levels were determined by atomic absorption spectrophotometer method. A significant difference was found between the CRF patient and healthy control groups in terms of genotype distributions of IL-18 (− 607 C/A) and MMP-2 (− 1306 C/T) gene variations (p < 0.05). The significant difference was found between the patient and control groups in terms of serum copper and zinc levels and copper/zinc ratio (p < 0.05). The significant difference was detected between patient and control groups in terms of copper and zinc levels and copper/zinc ratio according to IL-18 (− 607 C/A), IL-18 (− 137 G/C), and MMP-2 (− 1306 C/T) gene variations and genotype distributions (p < 0.05). In addition, significant difference was determined in terms of serum copper and zinc levels and copper/zinc ratio according to haplotypes of IL-18 (− 607 C/A), IL-18 (− 137 G/C), and MMP-2 (− 1306 C/T) gene variations between patient and control groups (p < 0.05). In conclusion, evaluation of IL-18 (− 607 C/A, − 137 G/C) and MMP-2 (− 1306 C/T) gene variations and serum trace element levels together is extremely important in terms of obtaining important biomarkers in CRF early diagnosis and progression.