Biochemical properties of free and immobilized candida rugosa lipase onto Al 2O 3: A comparative study

YEŞİLOĞLU Y., Şit L.

Artificial Cells, Blood Substitutes, and Biotechnology, cilt.39, sa.4, ss.247-251, 2011 (SCI-Expanded, Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 39 Sayı: 4
  • Basım Tarihi: 2011
  • Doi Numarası: 10.3109/10731199.2010.533125
  • Dergi Adı: Artificial Cells, Blood Substitutes, and Biotechnology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.247-251
  • Anahtar Kelimeler: Adsorption, Al 2O 3, Candida rugosa lipase, Immobilization, Optimization, Stability
  • Trakya Üniversitesi Adresli: Evet

Özet

Lipase from Candida rugosa (CRL) was immobilized by physical adsorption onto Al 2O 3. The immobilization yield was more than 95% during 30 min. The properties of free and immobilized enzyme were also searched and compared. The optimum pH was shifted from 7 to 8.5 by immobilization of the enzyme. The maximum activity of the free and the immobilized enzymes occured at 35 and 40°C, respectively. © 2011 Informa Healthcare USA, Inc.