Investigation on the antioxidant activity of roots and stem of colchicum turcicum l.


KILIÇ İ., YEŞİLOĞLU Y., BAYRAK Y.

Asian Journal of Chemistry, cilt.26, sa.1, ss.5-9, 2014 (Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 26 Sayı: 1
  • Basım Tarihi: 2014
  • Doi Numarası: 10.14233/ajchem.2014.15160
  • Dergi Adı: Asian Journal of Chemistry
  • Derginin Tarandığı İndeksler: Scopus
  • Sayfa Sayıları: ss.5-9
  • Anahtar Kelimeler: Antioxidant activity, Colchicum turcicum L, DPPH, Total flavonoids, Total phenol content
  • Trakya Üniversitesi Adresli: Evet

Özet

Water and acetone extracts from Colchicum turcicum L. were investigated for their antioxidant and radical scavenging activities in four different assays, namely, total antioxidant activity, 1,1-dipenyl-2-picryl-hydrazyl (DPPH) free radical scavenging, hydrogen peroxide scavenging and ferrous chelating activities. Antioxidant activity, ferrous chelating and hydrogen peroxide scavenging activities of C. turcicum extracts showed dose dependence and increased with concentration of C. turcicum extract. At the same concentration (100 μg/mL), water extract of C. turcicum (WECT) and acetone extract of C. turcicum (AECT) showed 53 and 48 % inhibition of lipid peroxidation of linoleic acid emulsion, respectively. On the other hand, butylated hydroxyanisole, butylated hydroxytoluene and ascorbic acid indicated inhibitions of 77, 71 and 59 % on the peroxidation of linoleic acid emulsion, respectively, at 50 μg/mL. Also, the total phenolic content in both water extract of C. turcicum and acetone extract of C. turcicum were determined as catechol equivalents. The total phenolics content of water and acetone extracts were determined by the Folin-Ciocalteu procedure and 0.454 and 2.172 mg catechol equivalent of phenols was detected in 1 g water extract of C. turcicum and acetone extract of C. turcicum.