Polyvinyl alcohol-trypsin as a catalyst for amino acid ester synthesis in organic media

YEŞİLOĞLU Y., KILIÇ İ.

Preparative Biochemistry and Biotechnology, cilt.34, sa.4, ss.365-375, 2004 (SCI-Expanded, Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 34 Sayı: 4
  • Basım Tarihi: 2004
  • Doi Numarası: 10.1081/pb-200031028
  • Dergi Adı: Preparative Biochemistry and Biotechnology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.365-375
  • Anahtar Kelimeler: Bovine trypsin, Enzymatic reaction, Ester synthesis, Organic solvent, Water activity
  • Trakya Üniversitesi Adresli: Evet

Özet

The bovine trypsin-catalyzed synthesis of N-α-benzoyl-DL-arginine esters from N-benzoyl-DL-arginine were studied in various organic solvents. Trypsin was immobilized to polyvinyl alcohol (PVA) by adsorption from its aqueous solutions. Immobilized enzyme showed higher catalytic activities than free enzyme for amino acid esterification in ethanol. The yield of ester is strongly dependent upon the PVA/trypsin ratio and water content in the reaction medium. The rate and equilibrium constant of the ester formation reaction are also dependent on water content.