Acute effect of thymoquinone on action potential and ionic currents of rat cardiac myocytes
BRATISLAVA MEDICAL JOURNAL-BRATISLAVSKE LEKARSKE LISTY, cilt.122, sa.6, ss.424-431, 2021 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 122 Sayı: 6
- Basım Tarihi: 2021
- Doi Numarası: 10.4149/bll_2021_070
- Dergi Adı: BRATISLAVA MEDICAL JOURNAL-BRATISLAVSKE LEKARSKE LISTY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, EMBASE, MEDLINE
- Sayfa Sayıları: ss.424-431
- Trakya Üniversitesi Adresli: Evet
Özet
OBJECTIVES: This study aims to investigate the acute effects of thymoquinone (TQ) which has been suggested to be a cardioprotective agent, on ventricular myocytes. METHODS: Freshly isolated rat ventricular myocytes were exposed to TQ and using standard whole-cell patch-clamp technique action potential (AP), sodium current (I-Na), L-type calcium current (I-CaL) and transient outward potassium current (I-to) were measured. RESULTS: TQ prolonged the duration of AP and decreased the peak value compared to that of control myocytes. Consistently, it inhibited I-Na in a concentration-dependent manner and shifted the channel kinetics to more hyperpolarized voltages. Besides, TQ not only inhibited I-to and I-CaL but also significantly attenuated the isoproterenol-induced increase in I-CaL. CONCLUSION: The effect of TQ on cardiomyocytes has been demonstrated for the first time. TQ changes AP morphology along with ionic currents and alleviates beta-adrenergic response in adult ventricular myocytes. These results indicate that TQ may be considered as a therapeutic agent in cases such as diabetic cardiomyopathy and cardiac hypertrophy, wherein the beta-adrenergic system is over-activated (Tab. 2, Fig. 6, Ref. 30).