INVESTIGATION OF ANTIOXIDANT ACTIVITY OF Sorbus domestica L. EXTRACTS AND DETERMINATION OF PHENOLIC CONTENTS BY LC-MS/MS


Ceylan B., YEŞİLOĞLU Y.

Revue Roumaine de Chimie, cilt.67, sa.6-7, ss.343-351, 2022 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 67 Sayı: 6-7
  • Basım Tarihi: 2022
  • Doi Numarası: 10.33224/rrch.2022.67.6-7.01
  • Dergi Adı: Revue Roumaine de Chimie
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.343-351
  • Trakya Üniversitesi Adresli: Evet

Özet

In this work, antioxidant activity of the extract Sorbus domestica L. was investigated using different methods. Phenolic contents were assessed using liquid chromatography-tandem mass spectroscopy (LC-MS/MS) technique. Antioxidant activity of S. domestica L. for different radical and compound was assessed. As a result, mountain S. domestica L. water extract showed the best antioxidant effect among other plant extracts tested. According to total antioxidant determination by thiocyanate method, water extract has the highest total antioxidant activity (23.54 mg ascorbic acid g-1 extract) compared to other extracts. The concentrations of the extracts providing 50% inhibition (IC50 value) of 2,2’-azino-bis(3-ethylbenzothioazoline-6-sulphoic acid) diammonium salt (ABTS•+) radical scavenging activity are between 9.14±0.50 and 10.97±0.25 μg mL-1. It was also seen that extracts exhibit good antioxidant activity; therefore, it can be claimed that S. domestica L. can be used as natural antioxidant source.