Akademik Veri Yönetim Sistemi
Turkish Journal of Thoracic and Cardiovascular Surgery, cilt.27, sa.1, ss.73-79, 2019 (SCI-Expanded, Scopus, TRDizin)
Background: This study aims to evaluate the effect of mitomycin-C applied through different drug administration approaches on the development of granulation tissue in the field of surgery and renal functions in rats which underwent tracheal surgery. Methods: Fifty male adult Sprague Dawley rats (weighing mean 200 g to 300 g) were divided into five groups. An incision was performed between the fifth and sixth cartilage ring of the trachea in all groups under anesthesia and the incision was primarily repaired with a 6/0 monofilament absorbable suture. A single dose of mitomycin-C 0.5 mg was applied in the experimental animals appropriate with their assigned groups as topical, intraperitoneal injection, injection to the wound edges, and through inhalation. No mitomycin-C was administered in one group which was accepted as the control group. Rats were sacrificed four weeks after surgery and their tracheas were excised subsequently. Tracheal tissue samples were histopathologically evaluated in terms of epithelization, fibrosis, amount of fibroblasts, angiogenesis, and inflammatory response. Diameter and wall thickness of the tracheas were measured. Blood urea and creatinine levels were evaluated for nephrotoxicity, and the rats were immunohistochemically examined for glomerular pathology. Results: Epithelization was statistically significantly decelerated (p < 0.01), diameter of the trachea was statistically significantly larger (p < 0.05), and wall thickness of the trachea was significantly thicker in the group with topical mitomycin-C application compared to the control group (p < 0.01). Conclusion: Topically applied mitomycin-C following tracheal surgery slows down epithelization and, thus, decreases the development of granulation tissue and maintains a wider diameter of the trachea. Amaç: Bu çalişmada trakea cerrahisi uygulanan siçanlarda farkli ilaç uygulama yaklaşimlari ile uygulanan mitomisin-C'nin cerrahi bölgede granülasyon dokusu gelişimi ve böbrek fonksiyonlari üzerine etkisi değerlendirildi. çalişma plani: Elli erkek erişkin Sprague Dawley siçan (ağirlik ort. 200 g-300 g) beş gruba ayrildi. Tüm gruplara anestezi altinda trakeanin beşinci ve altinci kikirdak halkasi arasindan kesi yapildi ve kesi 6/0 monofilaman emilebilir dikiş ile primer olarak onarildi. Deney hayvanlarina atandiklari topikal, intraperitoneal enjeksiyon, yara kenarlarina enjeksiyon ve inhalasyon yolu gruplarina uygun olarak bir doz 0.5 mg mitomisin-C uygulandi. Kontrol grubu kabul edilen bir gruba mitomisin-C uygulanmadi. Cerrahiden dört hafta sonra siçanlar sakrifiye edildi ve takiben trakealari çikarildi. Trakea dokulari histopatolojik olarak epitelizasyon, fibrozis, fibroblast miktari, angiogenezis ve enflamatuar yanit açisindan değerlendirildi. Trakea çapi ve duvar kalinliği ölçüldü. Kan üre ve kreatinin değerleri nefrotoksisite açisindan değerlendirildi ve siçanlar immunhistokimyasal olarak glomeruler patoloji açisindan incelendi. Bulgular: Kontrol grubuna kiyasla topikal mitomisin-C uygulanan grupta epitelizasyon istatistiksel olarak anlamli derecede yavaşladi (p < 0.01), trakea çapi istatistiksel olarak anlamli derecede daha genişti (p < 0.05) ve trakea duvar kalinliği anlamli olarak daha kalindi (p < 0.01). Sonuç: Trakea cerrahisi sonrasi topikal olarak uygulanan mitomisin-C epitelizasyonu yavaşlatarak granülasyon dokusu gelişimini azaltmakta ve trakea çapinin daha geniş kalmasini sağlamaktadir.