Entrapment of laurel lipase in chitosan hydrogel beads

YAĞAR H., Balkan U.

Artificial Cells, Nanomedicine and Biotechnology, cilt.45, sa.5, ss.864-870, 2017 (SCI-Expanded, Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 45 Sayı: 5
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1080/21691401.2016.1182920
  • Dergi Adı: Artificial Cells, Nanomedicine and Biotechnology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.864-870
  • Anahtar Kelimeler: Chitosan, immobilization, ionotropic gelatin method, laurel, lipase, tripolyphosphate
  • Trakya Üniversitesi Adresli: Evet

Özet

Laurel seed lipase was entrapped within chitosan beads with ionotropic gelatin method using tripolyphosphate (TPP) as multivalent covalent counter ion. Immobilization yield was 78%. First, optimum immobilization conditions were determined, and morphology of chitosan beads was characterized by scanning electron microscopy. Optimum pH and temperature were evaluated as 6.0 and 40 °C, respectively. The immobilized beads saved about 55% of its activities at 60° while saved about 32% at 70 °C for 30 min. Vmax/Km values were determined as 31.75 and 2.87 using olive oil as substrate for immobilized beads and free enzyme, respectively. Immobilized beads showed the activities during 30 days at +4 °C.