Koenzim Q10'un biyoyararlanımını arttırmak için proniozomal formülasyonların geliştirilmesi: İn vitro ve in vivo değerlendirilmeleri

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Thesis Type: Postgraduate

Institution Of The Thesis: Trakya University, SAĞLIK BİLİMLERİ ENSTİTÜSÜ, ., Turkey

Approval Date: 2023

Thesis Language: Turkish

Student: ŞENCAN BALCI

Supervisor: AHMET DOĞAN ERGİN

Open Archive Collection: AVESIS Open Access Collection

Abstract:

Coenzyme Q10 is one of the most important antioxidants produced by the body. It also functions as an electron carrier and is generally administered orally, but can also be applied to the body transdermally, orally, and ocularly. Coenzyme Q10 interacts with oxygen-derived free radicals to prevent damage to biomolecules. Therefore, it serves as a potent antioxidant in the body, contributing to the renewal of other antioxidants, cell signaling, cell growth, and gene expression. Additionally, it plays a significant role in membrane stability, energy conversion, and ATP production. Due to its low solubility and low bioavailability, the use of coenzyme Q10 is limited. As part of our thesis work, characterization studies were conducted on niosomal formulations obtained from proniosomes, and the identified formulas were subjected to in vivo pharmacokinetic studies. After the application of pure coenzyme Q10 and coenzyme Q10 -loaded proniosomes, blood samples were collected from rats at specified intervals over 24 hours, and the necessary measurements were performed. When pharmacokinetic parameters were examined, it was observed that the proniosome formulation exhibited effective bioavailability compared to the pure active substance. Quantitative determinations were carried out by developing an analytical method using the ICH reference in HPLC, and the validation of this method was performed. In this study, coenzyme Q10 was loaded into proniosomes, and because of in vitro and in vivo experiments, it was observed that the bioavailability of the active substance increased. In conclusion, our study has demonstrated that proniosome formulations are effective carriers in increasing bioavailability. Keywords: Slurry method, coenzyme Q10, proniosome, bioavailability, pharmacokinetics.